Skip to main content
. 2023 Jun 13;21(6):e3002097. doi: 10.1371/journal.pbio.3002097

Fig 2. DYRK1A regulates expression of ACE2 and DPP4.

Fig 2

(A) Immunoblot for ACE2 in WT Vero-E6 and DYRK1A KO cells. (B) mRNA abundance of ACE2 and DPP4 transcripts in WT Vero-E6 and DYRK1A KO clones assessed by qPCR. ΔΔCT values are calculated relative to actin and normalized to WT Vero-E6 values. (C) WT Vero-E6, DYRK1A KO#1, and DYRK1A KO#1 overexpressing recombinant hACE2 were infected with VSVpp-Rluc-SARS2-S, and % entry was assessed at 24 hpi. Immunoblot confirms rescue of ACE2 expression in DYRK1A KO#1. (D) WT Vero-E6, DYRK1A KO#2, and DYRK1A KO#2 overexpressing recombinant hDPP4 were infected with VSVpp-Rluc-MERS-S, and % entry was assessed at 24 hpi. Immunoblot confirms DPP4 overexpression in DYRK1A KO#2. % Entry in (C) and (D) was normalized to VSVpp-Rluc-VSV-G and WT Vero-E6 cells. Data were analyzed by unpaired Student t test; ns p > 0.05, * p < 0.05, ** p < 0.01, *** p < 0.001. Shown are means ± SEM. Each data point is the mean of 3–5 technical replicates. All experiments were performed at least 3 independent times. Data underlying this figure can be found in S1 Data and S1 Raw Images. ACE2, angiotensin-converting enzyme 2; DPP4, dipeptidyl peptidase-4; DYRK1A, Dual Specificity Tyrosine Phosphorylation Regulated Kinase 1A; hACE2, human ACE2; hDPP4, human DPP4; hpi, hours postinfection; KO, knockout; Rluc, Renilla luciferase; VSVpp, VSV pseudovirus; WT, wild-type.