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. 2023 Jun 13;21(6):e3002097. doi: 10.1371/journal.pbio.3002097

Fig 5. The proviral role of DYRK1A is conserved in human lung epithelial cells.

Fig 5

(A) Polyclonal KOs of DYRK1A were generated in Calu-3 human lung epithelial cells with 2 independent guides (sgRNA#1 and sgRNA#2). Viral titers were assessed after 24 hpi by TCID50 and compared against an NTG. (B, C) Scatter plots correlating DYRK1A and ACE2 mRNA levels in healthy human lung tissue [94,95]. Data in (A) are means ± SEM from 5 independent experiments. (A) Data were analyzed by unpaired Student t test; ** p < 0.01 or (B, C) Pearson and Spearman correlation statistical tests. Data underlying this figure can be found in S1 Data. ACE2, angiotensin-converting enzyme 2; DYRK1A, Dual Specificity Tyrosine Phosphorylation Regulated Kinase 1A; hpi, hours postinfection; KO, knockout; NTG, nontargeting guide.