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. 2023 Mar 27;120(4):e20220169. [Article in Portuguese] doi: 10.36660/abc.20220169

Gene expression and western blot results of the patients with CAD. Membrane images of PTEN and P-PTEN protein levels determined using specific antibodies in myocardial tissues of female/male patients (A). PTEN and P-PTEN protein expression findings in myocardial tissue taken from patients before and after surgery. β-actin was used as a loading control (B). The expression of the PTEN gene was determined by quantitative real-time PCR (qRT-PCR) in myocardial tissues taken from patients at 2 different times (C). Expression of the PTEN gene was determined by quantitative real-time PCR (qRT-PCR) in blood samples taken from patients at 2 different times and in control blood samples (D). Comparisons: P-PTEN BO vs. P-PTEN AO, PTEN BO vs. PTEN AO, and PTEN-T1 vs. PTEN-T2 (in blood and tissue samples separately) via Wilcoxon Signed Rand test; PTEN-T1 vs. Control and PTEN-T2 vs. Control via Mann-Whitney U test (*p<0.05, **p<0.001). (T1: before cardiopulmonary bypass, T2: after cardiopulmonary bypass).

Gene expression and western blot results of the patients with CAD. Membrane images of PTEN and P-PTEN protein levels determined using specific antibodies in myocardial tissues of female/male patients (A). PTEN and P-PTEN protein expression findings in myocardial tissue taken from patients before and after surgery. β-actin was used as a loading control (B). The expression of the PTEN gene was determined by quantitative real-time PCR (qRT-PCR) in myocardial tissues taken from patients at 2 different times (C). Expression of the PTEN gene was determined by quantitative real-time PCR (qRT-PCR) in blood samples taken from patients at 2 different times and in control blood samples (D). Comparisons: P-PTEN BO vs. P-PTEN AO, PTEN BO vs. PTEN AO, and PTEN-T1 vs. PTEN-T2 (in blood and tissue samples separately) via Wilcoxon Signed Rand test; PTEN-T1 vs. Control and PTEN-T2 vs. Control via Mann-Whitney U test (*p<0.05, **p<0.001). (T1: before cardiopulmonary bypass, T2: after cardiopulmonary bypass).