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. Author manuscript; available in PMC: 2023 Jun 14.
Published in final edited form as: Cell Rep. 2021 Jan 5;34(1):108573. doi: 10.1016/j.celrep.2020.108573

Figure 3. Single-Cell-Level Developmental Stage Scoring Places Individual UCB Naive T Cells Intermediate between Fetal and Adult.

Figure 3.

Fetal splenic, full-term UCB, and adult PB naive CD4 (n = 15) and CD8 (n = 13) T cell samples were profiled by single-cell RNA sequencing (scRNA-seq). See also Figures S3 and S4.

(A) UMAP plot showing distribution of cells of each age and CD4-versus-CD8 lineages. Separate plots along the bottom and right show cells of individual identities.

(B) UMAP plot showing Louvain clustering of single-cell profiles.

(C and D) Quantification of CD4 (C) and CD8 (D) T cell clustering where each column represents a different sample. A, adult; F, fetal; U, UCB.

(E) Overview of single-cell developmental stage score model generation.

(F and G) Developmental stage scoring of individual CD4 (F) and CD8 (G) naive T cells for each sample. Points are individual cells.