Figure 7. Single-Cell Developmental Stage Scoring Places UCB HSPCs Intermediate between Fetal and Adult.
CD34+ HSPCs from fetal bone marrow (BM) (n = 3), full-term UCB (n = 2), and adult BM (n = 2) samples were profiled by single-cell RNA-seq. Fetal-to-adult transition phenotype of cells were developmental stage scored, separately for each annotated cell type. See also Figures S3 and S6.
(A) UMAP plot showing cells of each age after batch correction
(B) Left: UMAP plot showing final cell type annotations with inferred cluster-based differentiation trajectories overlaid on top. Right: tree diagram showing accepted hematopoietic differentiation trajectories leading to all cell types annotated in this dataset. CLP, common lymphoid progenitor; CMP, common myeloid progenitor; GMP, granulocyte-monocyte progenitor; HSC, hematopoietic stem cell; MEP, megakaryocyte-erythroid progenitor; MPP, multipotent progenitor.
(C) Percentages of age identities of cells annotated as each cell type. N/A refers to cells not annotated in trajectory-end cell type clusters.
(D) Percentages of cell type annotations per sample. p values from chi-square test, **p < 1 × 10−15.
(E–G) Developmental stage scoring of individual HSC/MPP (E) MEP (F), and GMP (G) cells for each sample. Points are individual cells. Fetal and adult cells used to build developmental stage score models are not included. p values from Mann-Whitney U test, **p < 1 × 10−15.