Figure 5.

Sequence analysis of insert junctions in several mutants. Insertion of the ura4⁺ cassette (blue text) into genomic DNA (red and black text) is shown. (Left) Genomic sequences at upstream junction; (right) genomic sequences at downstream junction; (center) inserted ura4⁺ cassette sequences with terminal nucleotides and their positions (superscript) after integration. The red and black text flanked by deltas below the blue insert sequences are the genomic sequences deleted in each event. Nucleotide identity between the integrated construct and genomic DNA is displayed in red. Bottom line, the ura4⁺ cassette (blue) showing the terminal 13 nt at each end and the random tails (N18) upstream (left) and downstream (right). The top five mutants were generated by the ura4⁺ cassette flanked by degenerate tails while the bottom strain was mutagenized by the GFP cassette. Linker, upstream linker of the GFP cassette. The rad24-i1 strain contained a 1589 bp genomic deletion which included half of the 3′-end of the rad24⁺ gene (nucleotide positions +568 to +1283) and sequences downstream.