Figure 1.

Overview of presence of COPII, SEA/GATOR/NPC and hypothetical (Hypo) subunits/proteins in Euglenozoa. Proteins pulled down with PpSec13a or PpSec13b proteins or both are shown in orange, blue or green, respectively. The annotation of sequences was confirmed by reverse best hit and/or phylogenetic analyses. The only notable exception was B. saltans Sea3 (marked by asterisk), which retrieved Sea4 in BLAST against P. papillatum proteins, but Sea3 against heterolobosean N. gruberi (electronic supplementary material, table S7). In the phylogenetic tree, it clustered together with Sea3 sequences from trypanosomatids, and thus we concluded that it is in fact Sea3 as well. For individual phylogenetic trees, see electronic supplementary material, figures S1 and S2.