Fig. 1. Induction of hepatic lipid accumulation and hypertriglyceridemia by TAC. (a, b) Representative ORO and HE staining results of liver sections after TAC treatment for eight weeks. (c‒f) TG, TC, HDL-c, and LDL-c levels in mouse plasma. (g‒i) TG contents of the three cell lines (HepLi-2, HepG2, and AML12) after TAC treatment for 48 h. (j) Representative ORO staining results of the three cell lines. Scale bar=20 μm. Data are shown as mean±SD, n=3. ^* P<0.05, ^** P<0.01, ^*** P<0.001; n.s.: not significant. HE: hematoxylin-eosin; ORO: Oil Red O; TAC: tacrolimus; NC: negative control; TC: total cholesterol; TG: triglyceride; LDL-c: low-density lipoprotein cholesterol; HDL-c: high-density lipoprotein cholesterol; SD: standard deviation.