Fig. 2. Induction of lipid accumulation by TAC through autophagy inhibition. (a) Immunoblot detection of LAMP1, TFEB, P62, LC3B, and nTFEB in HepG2 and AML12 cell lines. (b) Representative TFEB staining results of the two cell lines. (c) Representative LC3B staining results of the two cell lines. (d) Representative LAMP1 and lipid-droplet staining results of the two cell lines. Scale bar=10 μm. TAC: tacrolimus; NC: negative control; LAMP1: lysosomal-associated membrane protein 1; TFEB: transcription factor EB; P62: protein 62; LC3B: microtubule-associated protein 1 light chain 3β; nTFEB: nuclear TFEB; H3: histone 3; DAPI: 4',6-diamidino-2-phenylindole.