Fig. 3. Reversion of TAC-induced lipid accumulation by overexpression of FGF21. (a) Immunoblot detection of FGF21 expression in the liver. (b) qPCR-assisted detection of FGF21 in the liver. (c) FGF21 serum level. (d) Immunoblot detection of FGF21 expression in three cell lines. (e) qPCR-assisted detection of FGF21 in three cell lines. (f) Immunoblot detection of FGF21 overexpression in three cell lines. (g) qPCR-assisted detection of FGF21 overexpression in three cell lines. (h) TG contents after DMSO or TAC treatment in three cell lines with or without FGF21 overexpression. (i) Representative ORO staining results of three cell lines. Scale bar=20 μm. Data are shown as mean±SD, n=3. ^* P<0.05, ^** P<0.01, ^*** P<0.001. ORO: Oil Red O; FGF21: fibroblast growth factor 21; TAC: tacrolimus; NC: negative control; OE: overexpression; qPCR: quantitative real-time polymerase chain reaction; TG: triglyceride; DMSO: dimethyl sulfoxide; mRNA: messenger RNA; SD: standard deviation.