Fig. 5. Effect of recombinant FGF21 protein on TAC-impaired autophagy. (a) Immunoblot detection of LAMP1, TFEB, P62, LC3B, and nTFEB in mouse livers. (b) Representative TFEB staining results of liver sections. (c) Representative LC3B staining results of liver sections. (d) Representative LAMP1 and lipid-droplet staining results of liver sections. (e) Immunoblot detection of LAMP1, TFEB, LC3B, and nTFEB in HepG2 and AML12 cell lines. (f) Representative LC3B staining results of AML12 cell line. (g) Representative LAMP1 and lipid-droplet staining results of the two cell lines. Scale bar=10 μm. FGF21: fibroblast growth factor 21; LAMP1: lysosomal associated membrane protein 1; TFEB: transcription factor EB; P62: protein 62; LC3B: microtubule-associated protein 1 light chain 3β; TAC: tacrolimus; NC: negative control; nTFEB: nuclear TFEB; rh(m)FGF21: recombinant human (mouse) FGF21; DAPI: 4',6-diamidino-2-phenylindole; H3: histone 3.