Fig. 3. IF and qRT-PCR results of IL-6 and IL-10 in the rats after scaffold implantation. (a) Representative images of IL-6 (green) and IL-10 (red) IF staining in the NAg-CCS and blank CCS groups on postoperative Days 3 and 7. (b, c) Cell counts of IL-6-positive (b) and IL-10-positive (c) macrophages in the NAg-CCS and blank CCS groups on Days 3 and 7 ( n=4). (d, e) Relative mRNA expression of IL-6 (d) and IL-10 (e) detected by qRT-PCR in the NAg-CCS and blank CCS groups on Days 3 and 7 ( n=3). Data are expressed as mean±standard deviation (SD). ^⁎⁎ P<0.01, ^⁎⁎⁎ P<0.001. IF: immunofluorescence; qRT-PCR: quantitative real-time polymerase chain reaction; IL: interleukin; NAg: nanosilver; CCS: collagen-chitosan scaffold; mRNA: messenger RNA; DAPI: 4',6-diamidino-2-phenylindole.