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. 2023 Jan 2;41(6):858–869. doi: 10.1038/s41587-022-01546-1

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a, Maximum intensity projection of a sagittal mouse brain section expanded with Magnify-ProK. Yellow, DiO; White, DAPI. EF = 4.5× in PBS. b, Zoom in of boxed region in a showing imaged field in subsequent panels. Endogenous tdTomato can be seen in dopaminergic neurons in the ventral tegmental area. Cyan, Lycopersicon esculentum Lectin; magenta, crimson-tdTomato; white, DAPI. c, Zoom in of boxed region in b showing individual channels. d, 3D reconstruction of merged panels from c. e, 3D reconstruction of an SST cell in a fully expanded mouse cortex expanded with Magnify and homogenized with hot surfactant solution. Endogenous SST–GFP signal was recovered with an anti-GFP antibody applied postexpansion. Yellow, anti-GFP; cyan, PSD95; magenta, synaptophysin; white, DAPI. Synaptic markers close to GFP signal have been highlighted. EF = 9× in ddH₂O. f, Zoom in of boxed region in e showing synapses on dendritic spines. g, Single z plane of a fully expanded SST neuron in mouse cortex from the same sample as e. h, Zoom in of boxed region in e showing synapses onto SST dendrite. Scale bars, a, 2.5 mm; b, 50 µm; c, 20 µm; d, 13 µm; (e,g, 5 µm; f,h, 2 µm. Scale bars are all in biological scale.