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. 2023 Jan 2;41(6):813–823. doi: 10.1038/s41587-022-01560-3

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 6 — (a) UMAP visualization of H3K4me1 and H3K27me3 (single signal and unmixed signal), colored by cell types derived from H3K4me1 and transferred to H3K27me3. Black rectangle indicates the B cell population used to re-cluster in (b,c,d). (b) UMAP of pro-B and B cells only. (c,d) Projection of H3K4me1 signal of marker genes for pro-B (c) or for differentiated B cells (d). H3K4me1 signal is measured in all cells of the H3K4me1 UMAP (that is both single- and double-incubated have H3K4me1 signal in the H3K4me1 UMAP). Double- (colored) but not single-incubated (grey) cells have H3K4me1 signal in the H3K27me3 UMAP.