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. 2023 Feb 23;72(7):2113–2125. doi: 10.1007/s00262-023-03409-3

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© The Author(s) 2023

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Experimental approach overview. B16-F10 cells were stimulated with INF-γ for 48 h. After that the SIINFEKL peptide was added. The expression of MHC-I and the presence of MHC-I/SIINFEKL complexes were analyzed by flow cytometry. Gray histogram: unstimulated B16-F10 cells; light blue histogram: MHC-I expression in IFN-γ treated cells; purple histogram: MHC-I/SIINFEKL on the surface of cells stimulated with IFN-γ + SIINFEKL peptide6