Fig. 4.

Effect of linker sequence in activation and proliferation of T cells. a Gating strategy for the analysis of CD8⁺ T cells activation. b Frequency of CD8⁺ CD25⁺ CD44⁺ T cells, 24-h post-co-culture of B16-F10 transfected cells with splenocytes from OT-I mice. c Gating strategy for the analysis of the CD8⁺ T cells proliferation. d Frequency of proliferating CD8⁺ T cells at 72-h post-co-culture of B16-F10 transfected cells with splenocytes from OT-I mice. Mean plus SD of three replicates is shown in all graphs. All data were analyzed using Kruskal–Wallis test corrected for multiple comparisons by original FDR method of Benjamini and Hochberg, ^*P < 0.1, ^**P < 0.01