Fig. 4. The effect of sex and diet on adipogenic capacity of visceral APCs in mice.

a Representative UMAPs from male or female mice demonstrating that Pdgfra⁺CD34^low cells are CD200^-. b Representative images showing lipid accumulation measured by BODIPY (red) and Dapi (blue) staining following differentiation of CD34^lowCD200^- visceral APCs in male or female mice fed either a normal chow (NC) diet or (HFD) for 8 weeks. c Quantification of positive BODIPY area normalized to Dapi. d, e Relative mRNA expression of Pparγ and Fabp4 in undifferentiated (undiff) or differentiated (diff) visceral APCs from female or male mice fed a NC diet. f Relative mRNA expression of Pparγ in undiff or diff visceral APCs from male mice fed NCD or HFD, respectively. g Relative mRNA expression of Pdgfra in undifferentiated visceral APCs from female or male mice fed NCD. Values are mean ± SD. n = 3 independent experiments. Scale bar in (b) is 50 µm.