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. 2023 Jun 13;6:638. doi: 10.1038/s42003-023-05011-w

Fig. 6. Bmper increases during differentiation of 3T3-L1 preadipocytes and is important for adipogenesis.

Fig. 6

a Relative mRNA expression of Bmper in 3T3-L1 cells during different timepoints of differentiation. b Relative mRNA expression of Pparγ in 3T3-L1 cells during different timepoints of differentiation. c Secreted Bmper protein measured during different timepoints of differentiation in 3T3-L1 cells. d Relative mRNA expression of Bmper following siRNA knockdown in 3T3-L1 cells at different timepoints of differentiation. e Oil red O staining and quantification in differentiated 3T3-L1 cells treated with control siRNA or Bmper siRNA knockdown. f Lipid content during differentiation in control or Bmper siRNA knockdown measured by BODIPY intensity. Values are means ± SD. n = two independent experiments.