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. 2023 Jun 13;6:638. doi: 10.1038/s42003-023-05011-w

Fig. 7. Bmper knockout in mouse primary visceral APCs reduces adipogenesis.

Fig. 7

a Schematic detailing the generation of Bmperfl/fl mice on a C57BL6/J background using CRISPR/Case 9. Adipose progenitor cells (APCs) were sorted from epididymal white adipose tissue of male Bmperfl/fl mice. b Representative images of green fluorescent protein (GFP) expression in visceral APCs from male Bmperfl/fl mice infected with adenovirus expressing control GFP (Ad-CM-GFP) or adenovirus expressing Cre-GFP (Ad-CMV-Cre-GFP) prior to differentiation. c Representative images showing lipid accumulation in APCs infected with control GFP or Cre-GFP vectors and stained with BODIPY (red) and Dapi (blue) following differentiation. d Quantification of positive BODIPY area normalized to Dapi. e–g Relative mRNA expression of Bmper, Pparγ, and Fabp4 in control GFP or Cre GFP following differentiation. Values are mean ± SD. n = three independent experiments.