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. 2023 Jun 13;14:3502. doi: 10.1038/s41467-023-38922-7

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a The centromeric Chio1 and Chio2 satellite arrays coincide with the enriched sites of CENH3. The LTR transposable elements (TEs), including Ty3/gypsy and Ty1/copia TEs, show uniform distribution in the (peri)centromeric and intercentromeric regions. Chromosome 4 is taken as a representative chromosome. b–e FISH on mitotic C. japonica chromosomes with different repeats. b CjSat3 shows a clustered distribution, while (c) CjSat4 and (d) CjSat5 show dispersed and subtelomeric localization, respectively. e The 45S rDNA locates at one end of a chromosome pair. Chromosomes were counterstained with DAPI. Scale bar, 5 µm. b–e At least two independent experiments were carried out to confirm the reproducibility of the labeling patterns.