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. 2023 Jan 18;8(5):479–496. doi: 10.1016/j.jacbts.2022.10.001

Figure 2.

Figure 2

NLRC5 Deletion Promoted Cardiac Remodeling Induced by Pressure Overload

(A) Gross morphology of hearts from wild-type (WT) and knockout (KO) mice after 4 weeks of transverse aortic constriction (TAC). (B) The ratios of heart weight and tibia length (HW/TL) in WT and KO mice (n = 10 per group). (C) Histological analysis of cardiomyocyte area was measured by wheat germ agglutinin staining. Scale bars, 30 μm. (D) Representative images of transverse area of cardiomyocytes detected by hematoxylin and eosin staining. The cross-sectional areas of cardiomyocytes were analyzed by ImageJ (NIH) (n = 10 per group). Ten fields per section from each sample are analyzed. (E) The mRNA expression of Nlrc5 was assessed in heart tissues, normalized to Gapdh (n = 10 per group). (F) Relative mRNA expression of cardiac hypertrophy–related markers Anp and Bnp was assessed (n = 10 per group). (G) Representative Western blots determined the protein expression of ANP and NLRC5 in WT and KO mice after 4 weeks of TAC operation (n = 10 per group). (H to M) Echocardiographic analyses of ejection fraction, fractional shorting, left ventricular internal diameter at end-systole (LVIDs), left ventricular posterior wall diameter at end-systole (LVPWs), and left ventricular (LV) mass after 4 weeks of TAC operation (n = 10 per group). (N) Representative Masson’s trichrome staining and quantitative analysis of collagen volume of hearts from WT and KO mice after sham or TAC operation (n = 10 per group). Scale bars, 50 μm. (O and P) Immunohistochemistry staining and quantitative analysis of cardiac fibrosis reflected by fibronectin and collagen I in WT and KO mice with sham or TAC operation (n = 10 per group). Scale bars, 50 μm. (Q) Relative mRNA expression of cardiac fibrosis markers Fibronectin and Collagen Iα was measured in heart tissues from WT and KO mice with sham or TAC operation (n = 10 per group). Data are expressed as mean ± SD. ∗P < 0.05, ∗∗P < 0.01. Data in E and F were analyzed using Kruskal-Wallis followed by Dunn’s multiple comparison test. Other data were analyzed using 1-way analysis of variance followed by Tukey’s post hoc analysis.