Figure 3.

Changes in Monocyte Counts, Subset Frequencies, and Lipid Accumulation

(A) Total monocyte counts. (B) Representative flow cytometric examples and frequencies of monocyte subsets: CD14⁺⁺/CD16^– classical (cM), CD14⁺/CD16⁺ intermediate (iM), and CD14^dim/CD16⁺ nonclassical (nM) monocytes. (C) Side scatter (SSC), representing cell granularity/lipid droplets, and Nile Red staining for lipids (indicated by relative mean fluorescence intensity [MFI] of Nile Red staining) of monocyte subsets. Values are mean ± SEM. ∗P ≤ 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001 for main effects; ^#P < 0.05 post hoc test for pairwise comparisons between diets at indicated time points. D5 = day 5; FSC = forward scatter; other abbreviations as in Figure 2.