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. 2023 May 31;14:1172758. doi: 10.3389/fpls.2023.1172758

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Copyright © 2023 Capriotti, Ricci, Molesini, Mezzetti, Pandolfini, Piunti and Sabbadini

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) Amplification of 35S promoter (340 bp) gene fragment from genomic DNA of nine transgenic lines (#A - #L) of Thompson Seedless (Th), one line (#H) of Ancellotta (An) obtained after A. tumefaciens infection, plus the wild-type controls. Positive control corresponds to pK7WG2-35S-eGFP-nptII PCR product. The lanes labelled “Negative control” show the PCR result using deionized sterile water. 1Kb ladder (Invitrogen™ by Thermo Fischer scientific). (B) Southern blot analysis of nptII copy number in grapevine transgenic plants. Genomic DNA from Thompson Seedless and Ancellotta wild-type and transgenic lines was digested with KpnI, which cuts T-DNA at a single position.