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. 2023 May 31;14:1186322. doi: 10.3389/fmicb.2023.1186322

Table 2.

Primers for complete genome amplification.

Name Primer sequence (5’-3’) Position in the genome Product size (bp)
HP-PRRSV-A ATGACGTATAGGTGTTGGCTCTATG
GAGCGGCTGGGATGGTACTGCTAGG
1–1,894 1894
HP-PRRSV-B GTGAGCATTGGACTGTCTCTGTGAT
ACATCCGGGGATCTTTGGCAGGTTG
1,700–3,620 1921
HP-PRRSV-C TTTGTGATGTTACCTCGCACGCCTG
ACCAATAACACCACGGCCAAGATTC
3,430–5,300 1871
HP-PRRSV-D ATCGGAGGCATGGCTCATAGGTTGA
GTGCTACCAACCTTTAGGTCGAAGA
5,106–7,010 1905
HP-PRRSV-E CTGCGTCCAACATGAGGAATGCAGC
CCGAGGGCGATGGGCGAGTTGAACG
6,780–8,700 1921
HP-PRRSV-F AGGCTGTGCGAGAAAACTGGCAAAC
TTTGTCCCTGTAATCTGGTTGAATG
8,550–10,410 1861
HP-PRRSV-G GGCTTCTCAGTAAAACAACTCTCAC
CAAACAAAATGGCCAAAAATATGAT
10,230–12,100 1871
HP-PRRSV-H GAGGACTGGGAGGATTACAATGATG
GTGCCAGCCAATCTGTGCCATTCAG
11,850–13,840 1991
HP-PRRSV-J TGCTTCATTTCATGACACCTGAGAC
TTTTTTAATTACGGCCGCATGGTTC
13,610–15,323 1714