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. 2023 May 31;10:1182550. doi: 10.3389/fvets.2023.1182550

Figure 1.

Figure 1

Passages of A/silver teal/Argentina/CIP051-32/2011(H4N2; 32/H4N2) in 3-days old chickens. Adaptation was attempted by serially passaging the 32/H4N2 virus in specified pathogen-free chickens. In passage 1, the pool of lung homogenates were utilized as inoculum for passage 2. Afterwards, the lung homogenate with the lowest Ct value within the group (red dots) was utilized as inoculum for the next group. The green dot represents the chicken from which the P5Ch32/H4N2 virus was characterized.