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. 2023 May 31;14:1188555. doi: 10.3389/fimmu.2023.1188555

Figure 4.

Figure 4

FPR2 inhibition prevents inflammation of the skin and enhances colonization by S. aureus. CFUs of S. aureus recovered from infected N/TERT-1 (A) treated either with USA300 +/- tBOC (10 µM) (left) or USA300 +/- WRW4 (1 µM) (right), or CFU of S. aureus recovered from infected PHK +/- WRW4 (1 µM) (B). Skin of tape stripped wild-type and Fpr2-/- were colonized for 24 h with S. aureus either with addition of WRW4 or PBS (C). Recovered S. aureus (D) and IL-1α (E), IL-1β (F) or MIP-2 (G) derived from supernatants of skin punches of S. aureus colonized skin. Model of colonization of human skin explants with S. aureus (H). Bacteria derived from human skin explants after S. aureus colonization in combination with DMSO or WRW4 (1 µM), (H, I). Data represent mean and SEM of five independent experiments of baseline-corrected data (A), of three independent experiments of three different donors (B) of three/four mice per group (WT) and three mice per group Fpr2-/- (D) six mice per group (WT) and three mice per group of Fpr2-/- (E–G), two tapes per mouse and of five independent experiments with skin explants from 5 donors (I). *P < 0.05; **P < 0.01, significant difference versus the indicated controls as calculated by paired or two-tailed Student’s t-tests (A, B, G, I) or Mann-Whitney test (D, E).