Table 3. Analysis of coding joint formation in stable scid transfectants by transient V(D)J recombination assay.

Sf19-derived cell line	Expression vector stably introduced	Experiment	Coding (pJH290)
			(AmpR + CamR/AmpR)	Ratea (%)
6-1	pCMV6 alone	1	0/30 528	<0.003
		2	0/66 336	<0.002
		3	1/36 672	0.003
		4	0/19 453	<0.005
		5	3/68 600	0.004
		6	0/48 216	<0.002
2-2	Wild-type DNA-PKcs	1	97/42 720	0.227
		2	136/83 520	0.163
		3	40/63 072	0.063
		4	51/50 372	0.101
		5	39/18 816	0.207
		6	53/23 422	0.226
2-9	Wild-type DNA-PKcs	1	20/46 944	0.043
		2	16/51 072	0.025
		3	23/41 520	0.058
		4	16/72 128	0.022
		5	8/30 331	0.026
		6	4/16 360	0.031

^aRate is the percentage of replicated substrate which underwent V(D)J recombination and is derived from the formula [Amp^R + Cam^R/Amp^R] × 100.