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. 2023 May 31;14:1166511. doi: 10.3389/fpls.2023.1166511

Figure 3.

Figure 3

Barley growth and proteomic analysis of roots and shoots of DGR and LGR plants. (A) Pictures of barley (GP) seedlings in rhizoboxes grown in Cocopeat_black were taken at 4, 6, 8, and 16 DAS, respectively. Seven grains were sown per rhizobox (here out of PVC). Scale = 0.5 cm. (B) Root and shoot length of plants grown for 16 DAS. Plants were harvested from the rhizobox and washed. Light grown roots (LGR), dark grown roots (DGR). All shoots were in light. Violin plots show the root and shoot size of roots grown in the light (n = 12) and dark (n = 12). ** represents ≤0.005, **** represents ≤0.0001. Yellow line represents the median. (C) Functional classification of significantly up- or downregulated proteins in the root and shoot upon root illumination. Indicated proteins are involved in RNA, ATP, and metal ion binding, in the oxidoreductase and defense activity, and cytoskeleton-related proteins. Annotation from outer to inner circle: 8 DAS_S_DGR vs. S_LGR; 8 DAS_DGR vs. LGR; 16 DAS_S_DGR vs. S_LGR; 16 DAS_DGR vs. LGR. DAS, days after sowing; S_DGR, shoot of dark grown root; S_LGR, shoot of light grown root; LGR, light grown roots; DGR, dark grown roots. Cocopeat_black refers to Cocopeat including activated charcoal.