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. 2023 May 31;14:1202235. doi: 10.3389/fpls.2023.1202235

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Copyright © 2023 Ye, Shrawat, Moeller, Rode, Rivlin, Kelm, Martinell, Williams, Paisley, Duncan and Armstrong

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Centrifugation-assisted Agrobacterium inoculation drastically increased transient GUS expression in wheat ME surfaces. Agrobacterium AB32/pMON97367 was used in this experiment. In all treatments, MEs were centrifuged for 30 min at 4 °C. The GUS staining was performed after co-culture. The centrifugation force used is labeled above the picture. The relative centrifugal force (RCF) is marked as x g (g-force). Mature scutellum (S), coleorhiza (Cr) and the remains of suspensor (RS) positions are marked.