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Removal of 2,4-D auxin and Silwet® L-77 surfactant drastically improves transformation frequency. AB32/pMON264386 ( Figure S1 ) was used for all transformation. Bars represent averages of TF% of biological replicates for each treatment. *A paired t-test was used to analyze TF improvement significance (p<0.05). For each treatment, >10,000 explants were used per treatment and each treatment had at least three biological reps.
