Figure 7.
Agrobacterium-mediated direct transformation of wheat mature embryos. (A) Purified wheat MEs ready for transformation. (B) Co-cultured MEs. (C) Solid delay medium # 3996 (The co-culture filter paper containing wheat mature embryos was directly transferred onto the top of the solid delay medium). (D) Selection of multiple buds/shoots on liquid selection medium, (E) Regeneration on liquid regeneration medium. (F) Rooting on solid rooting medium. (G) T0 transgenic events in plugs. (H) Mature T0 transgenic events in the green house.