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EVs loading strategies and delivery mechanism. Engineered EVs can recognize target cells via biomarkers consisting of EV‐sorting domains fused with selective proteins and loading cargo by electroporation, transfection, incubation, and sonication. EVs are loadable with exogenous therapeutic cargos, including A) drugs, B) RNAs, C) proteins, and D) CRISPR/Cas9, similar to mainstream drug delivery systems like E) polymeric nanoparticles, F) polymeric micelles, and G) liposomes.
