Figure 6.

Inhibition of tumor growth by Hy‐M‐Exo in vivo. A) Schematic diagram of the therapeutic study in C3H mice. B) The image of the tumor masses. C) Average and D) individual tumor growth curves of tumor‐bearing mice vaccinated with various nanovesicles. Mice were sacrificed when the tumor size of the PBS group exceeded 2000 mm³, and peripheral blood and tumor masses were collected to analyze immune responses. E) Representative flow cytometry plots of CD3⁺CD45⁺ TILs and FOXP3⁺CD25^highCD4⁺ Tregs in the tumor masses of mice. Quantitative analysis of F) FOXP3⁺CD25^highCD4⁺ Tregs and G) ratio of CD8⁺ Teff to Treg in tumor masses. Data were shown as mean ±  SEM (n = 4). Statistical analysis was performed using (C) two‐way ANOVA with Tukey's multiple comparisons test and (F, G) unpaired two‐tailed Student's t‐test. *p < 0.05, **p < 0.01, ***p < 0.001.