Figure 4.

Run-off in vitro transcription. (A) The transcripts synthesized by different RNAPs with different fragments as template. Lane 1, 8401 RNAP with EB fragment; lane 2, 8401 RNAP with ES fragment; lane 3, 8401 RNAP preincubated with anti-E.coli σ⁷⁰ antibody and with ES fragment; lane 4, R.leguminosarum 248 RNAP with ES fragment; lane 5, E.coli RNAP with ES fragment; lane 6, 8401 RNAP preincubated with anti-NodD antibody and with EB fragment; lane 7, 8401 RNAP with PE fragment; lane 8, 8401 RNAP with EB fragment; lane 9, E.coli RNAP with PE fragment. The size markers shown on the right are RNA transcripts produced by E.coli RNAP with the 180 bp PE fragment as template. In the absence of CRP–cAMP, E.coli RNAP initiated transcription from the lacZp2 start site, giving rise to an 80 nt transcript and a 180 nt read-through. (B) Restriction fragments as template. The numbers on the right indicate the size of each fragment.