Table 1:
I-BET 762 reduces lung carcinogenesis in A/J mice
| Control | I-BET 762 | I-BET 762 | |
|---|---|---|---|
| 60 mg/kg diet | 120 mg/kg diet | ||
| Inflated lungs: | |||
| # of mice/group | 28 | 12 | 11 |
| # of tumors/group | 348 | 118 | 41 |
| # of tumors/lung (% control) | 12.4 ± 0.6 (100%) | 9.8 ± 0.9 * (79%) | 3.7 ± 0.6 ** (30%) |
| # of tumors < 0.5 mm (% of total tumors) | 9 (2.5%) | 20 (17%) ** | 26 (63%) ** |
| # of tumors > 1 mm (% of total tumors) | 33 (9.5%) | 0** | 0ǂ |
| Slides: | |||
| # of slides/group | 56 | 24 | 22 |
| Average # of tumors/slide (% control) | 3.84 ± 0.25 (100%) | 2.25 ± 0.36 * (58.6%) | 0.86 ± 0.19 * (22.5%) |
| Average tumor size, mm3 (% control) | 0.33 ± 0.03 (100%) | 0.12 ± 0.02 * (35.9%) | 0.06 ± 0.01 * (17.2%) |
| Average tumor burden, mm3 (% control) | 1.26 ± 0.14 (100%) | 0.27 ± 0.06 * (21%) | 0.05 ± 0.01 * (3.9) |
| Histopathology: | |||
| Total # LL Grade (% of total tumors) | 4 (2%) | 15 (28%) ** | 6 (32%) *** |
| Total # LH/HL Grade (% of total tumors) | 102 (47%) | 32 (59%) | 12 (63%) |
| Total # HH Grade (% of total tumors) | 109 (51%) | 7 (13%) ** | 1 (5%) ** |
Note: Female A/J mice were injected i.p. with 0.32 mg vinyl carbamate and were randomized into either control group fed AIN-93G diet or treatment groups fed I-BET 762 in the diet, starting one week after initiation with vinyl carbamate. After 16 weeks of treatment, lungs were harvested. The tumor number, size and histopathology were assessed in a blinded fashion by two independent investigators. Results are presented as mean±SEM.
*,
p < 0.05 vs. control
**,
p < 0.001 vs. control
ǂ,
p = 0.078 vs. control.