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. 2023 May 3;24(6):2512–2521. doi: 10.1021/acs.biomac.3c00025

Figure 1.

Figure 1

Stretching device. (A) (Left) PDMS chamber consisting of a PDMS frame with pin holes to attach the uniaxial stretching device and a PDMS sheet (thickness: ∼200 μm) with embedded fluorescent beads. Biotinylated VIFs at two different densities are bound to the PDMS-supported lipid bilayer harboring biotinylated lipids via biotin–neutravidin–biotin linkages. (Right) SUVs without biotinylated lipids serve as lipid reservoirs during stretching. (B) Components of the uniaxial stretching device. The pre-stretcher holding the PDMS chamber is mounted on the stretching device. For AFM measurements, an insert is placed in the center of the stretching device to close the hole, and a glycerol bed is placed between the PDMS chamber and the insert to dampen external vibrations.