Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 May 3;24(6):2512–2521. doi: 10.1021/acs.biomac.3c00025

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2023 The Authors. Published by American Chemical Society

Permits the broadest form of re-use including for commercial purposes, provided that author attribution and integrity are maintained (https://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Fluorescence micrographs of a strained lipid bilayer (mp = 10 mm) composed of POPC/DOPE-biotin-cap/ATTO647-DOPE (96:3:1, n/n) on an oxidized PDMS surface in the presence of SUVs (POPC/ATTO488-DOPE, 99:1, n/n) serving as a lipid reservoir. (A) ATTO647-DOPE fluorescence, (B) ATTO488-DOPE fluorescence, and (C) superposition of (A) and (B) showing that the defects (A, black areas) are filled with the lipid material originating from the SUVs (B, green areas); scale bars: 10 μm. (D) Fluorescence recovery curve after photobleaching of ATTO647-DOPE and ATTO488-DOPE, respectively. A diffusion coefficient of 1.0 ± 0.2 μm² s^–1 and a mobile fraction of 74 ± 7% were obtained for ATTO647-DOPE (N = 15, Figure S3). For ATTO488-DOPE (N = 9), a diffusion coefficient of 2.2 ± 0.7 μm² s^–1 and a mobile fraction of 85 ± 7% were found.