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. 2023 May 31;24(6):2766–2776. doi: 10.1021/acs.biomac.3c00178

Figure 4.

Figure 4

Representative live-cell spinning disk confocal microscopy images. (a) Schematic demonstrating cholesterol–oligonucleotide insertion into cell membranes and subsequent impact on VLP internalization. (b) C166 or (c) HeLa cells were dosed with 5 nM Qβ-VLPs for 180 min or pretreated with 5 μM cholesterol-terminated anchor oligonucleotides for 15 min and washed prior to treatment with 5 nM Qβ VLPs for 180 min. Maximum intensity projections for z-stack confocal images are shown in (b) and (c). (d, e) Mean fluorescence intensity per cell from data such as in (b) and (c), respectively. Statistics were performed in Prism by two-way ANOVA with Tukey’s multiple comparison test; **** = p ≤ 0.0001; *** = p ≤ 0.001; ** = p ≤ 0.01; * = p ≤ 0.05.