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. 2023 May 31;24(6):2766–2776. doi: 10.1021/acs.biomac.3c00178

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© 2023 The Authors. Published by American Chemical Society

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Comparison of cell-surface anchoring of DNA complementary or noncomplementary to VLP-displayed DNA. C166 cells pretreated with 1 μM cholesterol-anchored oligonucleotides for 15 min, washed in PBS, and treated with 2.5 nM VLP-18-mer or VLP-30-mer for 90 min. Cell-anchored oligonucleotides were (a) complementary or (b) noncomplementary to VLP-displayed strands. (c) Mean fluorescence intensity per cell from data such as in (a) and (b). Statistics were performed in Prism by two-way ANOVA with Tukey’s multiple comparison test; ** = p ≤ 0.01; * = p ≤ 0.05.