Table 2. CuAAC between Fluorogenic Azide and Alkyne-Labeled Oligonucleotides, Promoted by Ligand THPTA, BimC4A, or BimC4Ea.
Extent of reactivity was determined by fluorescence maxima following CuAAC (λex = 404 nm, λem = 477 nm). Conditions: 100 μM coumarin azide, 20 μM alkyne, 10 mM sodium ascorbate, 40 °C, 1 h.
