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. 2023 May 31;24(6):2766–2776. doi: 10.1021/acs.biomac.3c00178

Table 2. CuAAC between Fluorogenic Azide and Alkyne-Labeled Oligonucleotides, Promoted by Ligand THPTA, BimC4A, or BimC4Ea.

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a

Extent of reactivity was determined by fluorescence maxima following CuAAC (λex = 404 nm, λem = 477 nm). Conditions: 100 μM coumarin azide, 20 μM alkyne, 10 mM sodium ascorbate, 40 °C, 1 h.