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. 2023 Jun 7;26(1):360. doi: 10.3892/etm.2023.12059

Figure 2.

Figure 2

Evaluation of the radiodynamic effect of 5-ALA under normoxic and hypoxic conditions using standard colony formation assays in lymphoma cells in vitro. (A) Surviving fraction of lymphoma cells after radiodynamic therapy with 5-ALA under normoxic and hypoxic conditions. Columns, mean (n=9); error bars, SE. Cells were pre-treated with 5-ALA and exposed to IR. The total dose of IR was 2 Gy in each group. (B) Representative culture plate images from a standard colony formation assay in lymphoma cells. Cells without 5-ALA before IR (top) and cells pre-treated with 0.3 mM 5-ALA before IR (bottom). Colonies were stained using a blue tetrazolium bromide solution. *P<0.05; **P<0.01. 5-ALA, 5-aminolevulinic acid; IR, ionizing irradiation.