Fig. 5.

Directly antagonizing the Wnt/β-catenin axis negatively impacts PD-L1 expression. A Western blots showing the expression of the indicated proteins in HT-29 CC cells with or without prestimulation (IFNγ 50 ng/mL, 24 h). IFNγ-stimulated cells were further exposed to XAV-939 (Wnt inhibitor, 10 µM) or Atezolizumab alone or both together. The blots are representative of three replicates, and the results are shown in the densitometry graphs. B Immunofluorescence images showing the active β-catenin level in IFNγ-stimulated SW480 cells subsequently treated with XAV-939 (XAV) or Atezolizumab alone or in combination (XAV + Atezolizumab). Bars, 10 μm or 2 µm, as indicated in the images. The magnified images are grayscale images (inset) of active β-catenin in the region of interest marked with the white dotted line. In all the western blot panels, GAPDH served as the loading control. MW, relative molecular weight expressed in kilodaltons (kDa). Mean ± SEM. *P < 0.05, ***P < 0.001, two-tailed unpaired t test