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Table 2.
HPLC conditions
| Time (min) | Acetonitrile (%) | Water (%) |
|---|---|---|
| 0 | 25 | 75 |
| 5 | 25 | 75 |
| 30 | 50 | 50 |
| 34 | 50 | 50 |
| 35 | 25 | 75 |
| 40 | 25 | 75 |
Detection wavelength: 205 nm; sample injection volume: 40 µL.