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Table 3.
HPLC conditions
| Time (min) | Acetonitrile (%) | Water (%) |
|---|---|---|
| 0 | 80 | 20 |
| 2 | 80 | 20 |
| 70 | 50 | 50 |
| 70.5 | 80 | 20 |
| 80 | 80 | 20 |
Detection wavelength: 210 nm; sample injection volume: 10 µL.