Fig. 3.

Loss of Fkbp5 leads to the largest structural changes when restricted to GABAergic neurons of the forebrain. Data from the T2*-weighted MRI scan revealed underlying structural consequences of loss of Fkbp5 in either glutamatergic or GABAergic neurons of the forebrain, with the largest and most significant changes in Fkbp5^Dlx male and female mice. For Fkbp5^Dlx male mice, large and strongly significant increases in GM volume were found in the bilateral hippocampus compared to Fkbp5^lox/lox controls, whereas GM volumes were significantly decreased in the caudoputamen and lateral ventricle. Female Fkbp5^Dlx mice had larger GM volumes in the thalamus than their Fkbp5^lox/lox controls and strongly significant smaller GM volumes in the somatosensory cortex, claustrum, and auditory cortex. In addition, a number of WM structures were altered such as the corpus callosum and some GM areas around the BNST, although these clusters did not survive FWE correction. Furthermore, Fkbp5^Nex male mice had significantly smaller GM volumes in bilateral regions of the motor cortex extending to the anterior cingulate area vs. Fkbp5^lox/lox controls and uncorrected increased GM volumes were found in the left caudoputamen, right piriform area, bilateral dorsal thalamus and left ventral subiculum. Female Fkbp5^Nex mice had decreased GM volumes in the right anteriormedial visual area. Scales represent Z-scores. Yellow-red scale: KO > WT, Greenblue scale: KO < WT. AC = Auditory Cortex, ACA = Anterior Cingulate Area, CC = Corpus Callosum, Cl = Claustrum, CP = Caudoputamen, Hipp = Hippocampus, LV = Lateral Ventricle, PiA = Piriform Area, PrR = Pretectal region, RC = Retrosplenial Cortex, SMC = Sensorimotor cortex, SS = Somatosensory Cortex, Sub = Subiculum, Th = Thalamus, VC = Visual Cortex.