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. 2023 May 29;19(9):2803–2816. doi: 10.7150/ijbs.81726

Figure 2.

Figure 2

TRIM26 interacts with PBX1 protein. (A) The coverage and unique peptides of PBX1 and TRIM26 in the affinity-purified PBX1-interacting proteins by tandem mass spectrometry (MS/MS). (B) A representative MS/MS spectrum of TRIM26. The b- and y-ions were annotated and the peptide sequence, charge state (z), MH+, and Δmass were shown. (C) HA-PBX1 and Myc-TRIM26 were co-transfected into HEK293T cells for 48 hrs, then cells lysates were subjected to IP and IB assays. (D) Cell lysates were subjected to IP/IB with specific TRIM26 or PBX1 antibody or IgG, followed by IB assays. (E) Schematic diagram of TRIM26 truncates. (F) TRIM26 and its truncates were co-transfected with Flag-PBX1 into HEK293T cells for 48 hrs, followed by cell lysate preparation and IP/IB assays.(G) TRIM26 and its truncates were transfected into A549 and H226 cells for 48 hrs, followed by cell lysate preparation and IP/IB assays.(H) A549 and H226 cells were immunostained with specific antibodies against PBX1 (red) and TRIM26 (green), and then counterstained with DAPI (blue) to show nuclei.