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. 2023 May 29;19(9):2803–2816. doi: 10.7150/ijbs.81726

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TRIM26 mediates PBX1 K48-linked ubiquitination. (A) Myc-PBX1, Flag-TRIM26 and HA-Ub plasmids were co-transfected into HEK293T cells followed by IP/IB assay as indicated. (B) Flag-PBX1, Myc-TRIM26, siTRIM26 and HA-Ub plasmids were co-transfected into HEK293T cells followed by IP/IB assay as indicated. (C) A549 and H226 cells were transfected with siRNA, 48hrs later cell lysates were subjected to IP/IB assays as indicated. (D) A Myc-TRIM26 plasmid was co-transfected with Flag-PBX1, HA-K48-Ub, HA-K63-Ub, HA-K48R, or HA-K63R into HEK293T cells followed by IP/IB assay as indicated. (E-F) A549 and H226 cells were transfected with TRIM26 plasmids for 48 hrs, followed by IP/IB assays as indicated. (G) A549 and H226 cells were transfected with siTRIM26 for 48 hrs, followed by IP/IB assays as indicated.