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. 2023 May 29;19(9):2803–2816. doi: 10.7150/ijbs.81726

Figure 5.

Figure 5

TRIM26 suppresses PBX1 transcriptional activity. (A-B) HEK293T cells were co-transfected with Flag-PBX1, PRE.Luci, β-gal and TRIM26 (A) or TRIM26∆R (B) plasmids for 48 hrs, cell lysates were prepared for luciferase activity measurement and IB assays. mean±SD (n=3). ***, P<0.001; ns, not significant. (C-D) NSCLC cell lines were transfected with TRIM26 plasmids (C) or siTRIM26 (D) for 48 hrs, followed by IB assays to evaluate the expression levels of PBX1 and its downstream gene RNF6. (E-F) PBX1 were knocked out from A549 by its specific sgRNA. Cells were then transfected with a TRIM26 plasmid for 48 hrs. Total RNA was extracted for RT-PCR against TRIM26, PBX1, RNF6 or GAPDH (E). Cell lysates were subjected to IB against specific proteins as indicated (F). DU145 lacking PBX1 was used as a control.