TRIM26 promotes NSCLC cell proliferation and migration in association with PBX1 degradation. (A, B) A549 cells were transfected with a TRIM26 plasmid while H226 cells were transfected with siTRIM26, followed cell proliferation assessment by MTT assay. mean±SD (n=3). ***, P<0.001, versus control. (C, D) Cell proliferation was evaluated by EdU incorporation assay in A549 and H226 cells after transfection of TRIM26 plasmids or siTRIM26. mean±SD (n=3). **, P< 0.01, ***, P< 0.001, versus control. (E, F) A549 cells were transfected with TRIM26 plasmids and H226 were transfected with siTRIM26, followed by colony formation assay. Compared with control, ***, P<0.001. (G, H) A549 and H226 cells were transfected with Myc-TRIM26 or siTRIM26 for 48 hrs, followed by transwell assays. mean±SD (n=3). **, P< 0.01, ***, P< 0.001, versus control.