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. 2023 May 29;19(9):2803–2816. doi: 10.7150/ijbs.81726

Figure 8.

Figure 8

TRIM26 promotes NSCLC tumor growth in nude mice by downregulating PBX1. (A) Representative NSCLC and their para-cancerous tissues were collected for IHC assay against TRIM26 (A). (B) The statistical analyses on TRIM26 in the IHC assays as shown in A. (C) NSCLC and the paired normal tissues were subjected to IB, the ratio of TRIM26/GAPDH were analyzed. (D) TRIM26 expression is negatively associated with overall survival of patients with NSCLC. (E) A549 cells infected with TRIM26 or GFP lentivirus were injected into nude mice subcutaneously. Tumors were excised at the end of the experiment. (F) H226 cells infected with sgTRIM26 or control sgRNA (NC) lentivirus were injected into nude mice subcutaneously. (G-H) Tumor weights were then measured at the end of each experiment. mean±SD (n=6). ***, P< 0.001, versus control group. (I) Tumor samples from the xenografts were subjected to IB analyses for TRIM26 and PBX1with specific antibodies. (J) Tumors from E-F were subjected to IHC against specific proteins as indicated. (K) The hypothesis of TRIM26 promotes NSCLC by inducing PBX1 degradation via the ubiquitin proteasome pathway.